Malt Extract Yeast Agar Peptone (MYPA)
What is MYPA?
MYPA Mix is the most nutritious gelatinous media to cultivate, isolate and manipulate fungi. That is due to Peptone, which is derivate from soybeans that supports vigorous mycelium growth and demonstrates the most efficient media for long-term maintenance of mushroom cultures (more than a year). The uses of MYPA include:
- Growing mushroom mycelium for expansion into grain spawn
- Long term storage
Why use Malt Extract Agar Yeast for growing mushroom culture?
Malt extract contains a high concentration of maltose, which provides a suitable environment for the growth of yeast and mould. That is due to its rich source of carbon, protein and nitrogenous compounds that are used as energy for fungi growth.
Agar-Agar is extracted from various seaweeds, such as gracilaria and gelidium (It contains no animal ingredients. Due to Agar’s unique gel property and stability, it is used as a firming and thickening agent for Petri dish experimentation.)
Yeast (S. Cerevisiae)
The strain species S. Cerevisiae is grown on sugar beet molasses, making it a natural source of readily assimilated amino acids and proteins. For mushrooms, this means a rich supplemented diet of dietary fibre, Vitamin D, and B Complex (Thiamine, Riboflavin, Niacin, Pantothenic Acid, Folic Acid, Biotin, Vitamin B6 and Vitamin B12).
Mycological Peptone is derived from Soybean plants, commonly used in Liquid culture recipes and agar recipes and also added to Substrate as a supplement.
Supplementation with Peptone not only stimulates the growth of mycelium but also dramatically improves the quality of the projected Mycelium Mat.
*Not for human consumption*
These quantities are measured based on 60*15mm size Petri dishes:
The number of plates made will vary according to pouring techniques; we recommend using just enough to cover the surface of the petri dish.
*Petri dish is not included.
- 21 gm makes 35Agar Plates (Use 500ml of hot water)
- 42 gm makes 70 Agar Plates (Use 1000ml of hot water)
- 63 gm makes 105 Agar Plates (Use 1500ml of hot water)
- 84 gm makes 140 Agar Plates (Use 2000ml of hot water)
- 168gm makes 280 Agar Plates (Use 4000ml of hot water)
- Dissolve your MYPA packet in the specified ratio of hot water (i.e.1000, ml water for 42 gm of MYPA) and stir thoroughly with a spoon. If you have a magnetic stirrer, this will mix the solution properly. The content needs to be poured slowly and not all at once.
- Autoclave or pressure cook for 20 minutes at 15 psi.
- Pour into Petri dishes when it reaches 50 degrees Celsius (Approximately hot enough to hold the flask/ jar with one hand), and let it sit for 10 minutes. Do not stir the solution after taking it out of Autoclave, as this would lead to air bubbles being trapped in this solution.
- Once the Petri dishes are set, inoculate them and wrap them with parafilm. Keeping the plates aside for a week is recommended to check for contamination.
- Petri dishes are best to be stored upside down to avoid condensation.
Do not have a pressure cooker? No problem, just message us, and we will send you detailed instructions on how to use the microwave for sterilising along with your purchase.
You would need a reagent bottle to make agar. If made in a Microwave, a 500ml reagent bottle would fit. If using a Steriliser, then a 1000ml reagent bottle should fit.
We have relied on this Mix to experiment at our lab using different varieties.