Description
Plant cellThidiazuron (TDZ) 1g Powder is a powerful plant growth regulator belonging to the diphenylurea class of synthetic cytokinins. It is widely used in plant tissue culture to promote shoot regeneration, organogenesis, and somatic embryogenesis.
Key Benefits
Simple and convenient to use
Highly effective in stimulating shoot multiplication from explants in micropropagation
Enhances tissue culture media such as MS medium
Helps overcome apical dominance
Essential for the successful micropropagation of certain plant species
How to Use Thidiazuron (TDZ) Powder
Follow these steps to prepare tissue culture media using PCTโs TDZ Powder:
Prepare the medium: Use PCTโs MS or DKW media, or another suitable medium depending on your plant species.
Add supplements: For every 1 L of medium, add approximately 30 g of sugar, 1 mL/L PPM, and either 3โ4 g/L gellan gum or 6โ8 g/L agar.
Incorporate hormones: Add the required amount of TDZ and any additional plant hormones, such as those from your prepared NAA stock solution.
Sterilize: Autoclave the medium for 20 minutes at 15 psi and 121 ยฐC.
Once sterilized, your medium is ready for use.
